Establishment and Application of Engineered NIH 3T3 Cell Line with Stable Human RAGE Expression
نویسندگان
چکیده
منابع مشابه
cloning and expression of cd19, a human b-cell marker in nih-3t3 cell line
background: cd19 is a pan b cell marker that is recognized as an attractive target for antibody-based therapy of b-cell disorders including autoimmune disease and hematological malignancies. the object of this study was to stably express the human cd19 antigen in the murine nih-3t3 cell line aimed to be used as an immunogen in our future study. methods: total rna was extracted from raji cells i...
متن کاملCloning and Expression of CD19, a Human B-Cell Marker in NIH-3T3 Cell Line
BACKGROUND CD19 is a pan B cell marker that is recognized as an attractive target for antibody-based therapy of B-cell disorders including autoimmune disease and hematological malignancies. The object of this study was to stably express the human CD19 antigen in the murine NIH-3T3 cell line aimed to be used as an immunogen in our future study. METHODS Total RNA was extracted from Raji cells i...
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Total protein and DNA extracted from histologically diagnosed normal nonmalignant and esophageal tumor tissues were used for analysis of polypeptides pattern by two-dimensional gel electrophoresis and DNA transforming activity in NIH 3T3 cell transfection assay, respectively. In comparison to normal tissues, eight polypeptides underwent down-regulation or disappeared, while seven polypeptid...
متن کاملPreparation of Proper Immunogen by Cloning and Stable Expression of cDNA coding for Human Hematopoietic Stem Cell Marker CD34 in NIH-3T3 Mouse Fibroblast Cell Line.
PURPOSE Transmembrane CD34 glycoprotein is the most important marker for identification, isolation and enumeration of hematopoietic stem cells (HSCs). We aimed in this study to clone the cDNA coding for human CD34 from KG1a cell line and stably express in mouse fibroblast cell line NIH-3T3. Such artificial cell line could be useful as proper immunogen for production of mouse monoclonal antibodi...
متن کاملEstablishment a CHO Cell Line Expressing Human CD52 Molecule
Background: CD52 is a small glycoprotein with a GPI anchor at its C-terminus. CD52 is expressed by Normal and malignant T and B lymphocytes and monocytes. There are detectable amounts of soluble CD52 in plasma of patients with CLL and could be used as a tumor marker. Although the biological function of CD52 is unknown but it seems that CD52 may be involved in migration and activation of T-cells...
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ژورنال
عنوان ژورنال: Pharmaceutical Fronts
سال: 2020
ISSN: 2628-5088,2628-5096
DOI: 10.1055/s-0040-1708511